fluorophore pe cy7  (Cell Signaling Technology Inc)

Journal: Cell Reports Medicine

Article Title: Circulating cancer-specific CD8 T cell frequency is associated with response to PD-1 blockade in Merkel cell carcinoma

doi: 10.1016/j.xcrm.2024.101412

Figure Lengend Snippet:

Article Snippet: TCF7 Antibody, clone C63D9, fluorophore PE-Cy7 , Cell Signaling Technology , Cat# 90511S; RRID: AB_3086656.

Techniques: Recombinant, Staining, Multiplex Assay, Software

Journal: Blood Advances

Article Title: An in vitro model of human hematopoiesis identifies a regulatory role for the aryl hydrocarbon receptor

doi: 10.1182/bloodadvances.2023010169

Figure Lengend Snippet: AHR activation suppresses critical genes and key transcription factors involved in B cell and dendritic cell development. (A) Dot plot of the average gene expression of TCDD-treatment induced differentially expressed genes in MPP (multipotent progenitor) cluster on days 7 and 14. All DEGs are associated with a Bonferroni adjusted P value <.05. (B) Density of cells along MPP to lymphoid cells trajectory and expression of the top 4 highly variable genes that are differentially expressed by TCDD treatment and are involved in the development of lymphoid cells. (C) Percent BCL11A protein–expressing cells in overall population from 5 independent experiments. (D) Percent BCL11A protein–expressing cells in CD10 + CD19 – cells of vehicle and TCDD-treated groups across days from 5 independent experiments. (E) Transcription factor activity of IRF8 (analyzed by SCENIC) in pDC (plasmacytoid dendritic cell) cluster. Statistical significance of differences in TF activity between treatments at any time point was calculated using a Wilcoxon rank sum test. ∗ P < .05. (F) Percent IRF8 protein–expressing cells in overall population from 5 independent experiments. (C-D,F) Protein expression was measured using flow cytometry. Error bars show mean ± standard error of the mean. Statistical significance of differences in percentage of cells between treatments at any time point was calculated using a 2-tailed paired t test. ∗ P < .05, ∗∗ P < .01, ∗∗∗ P < .001.

Article Snippet: For intracellular staining, fixed cells were permeabilized by incubating in eBioscience Foxp3/TF permeabilization buffer (Invitrogen) for 20 minutes and incubated with relevant antibodies (antihuman Ctip-1 [Fluorophore PE, clone NB600-261; Novus Biologicals], antihuman IRF8 [Fluorophore APC, clone V3GYWCH; Thermo Fisher Scientific] and antihuman AHR [Fluorophore PE-Cy7 / PE, clone FF3399; Thermo Fisher Scientific]) for 60 minutes.

Techniques: Activation Assay, Expressing, Activity Assay, Flow Cytometry

Journal: PLoS Neglected Tropical Diseases

Article Title: Wuchereria bancrofti -infected individuals harbor distinct IL-10-producing regulatory B and T cell subsets which are affected by anti-filarial treatment

doi: 10.1371/journal.pntd.0007436

Figure Lengend Snippet: Using flow cytometry, peripheral whole blood cells from endemic normals (EN; n = 54), latent (CFA+MF-; n = 41) and patent (CFA+MF+; n = 13) Wuchereria bancrofti -infected, lymphedema (LE, n = 50) and previously infected individuals (PI; n = 65) were analyzed for frequencies (%) of ( A ) CD19 + CD24 high regulatory B cells expressing ( B ) CD5 + CD1d high . Graphs show box whiskers with median, interquartile ranges and outliers. Statistical significances between the indicated groups were obtained after a Kruskal-Wallis-test followed by a Dunn`s multiple comparison post hoc analysis. In addition, Spearman correlations were performed between MF counts and ( C ) CD19 + CD24 high or ( D ) CD19 + CD24 high CD5 + CD1d high frequencies.

Article Snippet: Thereafter, cells were stained with combinations of fluorophore (FITC, PE, PE-Cy7, APC)-conjugated anti-human CD1d (clone 51.1), CD4 (clone RPA-T4), CD5 (clone UCHT2), CD19 (clone HIB19), CD24 (clone eBioSN3 (SN3 A5-2H10)), CD38 (clone HIT2), CD127 (clone eBioRDR5), FOXP3 (clone 236A/E7), HELIOS (clone 22F6), IL-10 (clone JES3-9D7), eBioscience TM monoclonal antibodies from Thermo Fisher Scientific and CD304 (Neuropilin-1, clone 12C2) monoclonal antibody from Biolegend.

Techniques: Flow Cytometry, Infection, Expressing, Comparison

Journal: PLoS Neglected Tropical Diseases

Article Title: Wuchereria bancrofti -infected individuals harbor distinct IL-10-producing regulatory B and T cell subsets which are affected by anti-filarial treatment

doi: 10.1371/journal.pntd.0007436

Figure Lengend Snippet: Freshly isolated peripheral whole blood cells (100μl/well) from endemic normals (EN; n = 54), latent (CFA+MF-; n = 41) and patent (CFA+MF+; n = 13) Wuchereria bancrofti -infected, lymphedema (LE, n = 50) and previously infected individuals (PI; n = 65) were cultivated in 10% FCS/RPMI-1640 medium (100μl/well) and left either ( A-C ) un-stimulated or ( D-F ) cultured with eBioscience ™ cell stimulation cocktail (PMA) for 4 hours at room temperature. Thereafter, peripheral blood cells were analyzed for frequencies (%) of CD19 + B cells expressing ( A, D ) CD5 + CD1d high and CD19 + CD5 + CD1d high regulatory B cells expressing ( B, E ) IL-10. Graphs show box whiskers with median, interquartile ranges and outliers. Statistical significances between the indicated groups were obtained after a Kruskal-Wallis-test followed by a Dunn`s multiple comparison post hoc analysis. In addition, Spearman correlations were performed between MF counts and frequencies of CD19 + CD24 high CD38 high IL-10 + which were either ( C ) un-stimulated or ( F ) PMA stimulated.

Article Snippet: Thereafter, cells were stained with combinations of fluorophore (FITC, PE, PE-Cy7, APC)-conjugated anti-human CD1d (clone 51.1), CD4 (clone RPA-T4), CD5 (clone UCHT2), CD19 (clone HIB19), CD24 (clone eBioSN3 (SN3 A5-2H10)), CD38 (clone HIT2), CD127 (clone eBioRDR5), FOXP3 (clone 236A/E7), HELIOS (clone 22F6), IL-10 (clone JES3-9D7), eBioscience TM monoclonal antibodies from Thermo Fisher Scientific and CD304 (Neuropilin-1, clone 12C2) monoclonal antibody from Biolegend.

Techniques: Isolation, Infection, Cell Culture, Cell Stimulation, Expressing, Comparison

Journal: PLoS Neglected Tropical Diseases

Article Title: Wuchereria bancrofti -infected individuals harbor distinct IL-10-producing regulatory B and T cell subsets which are affected by anti-filarial treatment

doi: 10.1371/journal.pntd.0007436

Figure Lengend Snippet: Analysed regulatory immune cell subsets and their corresponding flow cytometry markers.

Article Snippet: Thereafter, cells were stained with combinations of fluorophore (FITC, PE, PE-Cy7, APC)-conjugated anti-human CD1d (clone 51.1), CD4 (clone RPA-T4), CD5 (clone UCHT2), CD19 (clone HIB19), CD24 (clone eBioSN3 (SN3 A5-2H10)), CD38 (clone HIT2), CD127 (clone eBioRDR5), FOXP3 (clone 236A/E7), HELIOS (clone 22F6), IL-10 (clone JES3-9D7), eBioscience TM monoclonal antibodies from Thermo Fisher Scientific and CD304 (Neuropilin-1, clone 12C2) monoclonal antibody from Biolegend.

Techniques: Flow Cytometry, Marker